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<h2 class="hd hd-2 unit-title">Reference Summary</h2>
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<h3> <b> Anatomy of a nucleic acid </b> </h3>
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<p> Above is a diagram of a nucleic acid polymer. <br /> <em> Recall that each nucleotide is composed of a sugar, phosphate and base. </em> <br/> S = Sugar <br /> P = Phosphate <br /> B= Base. </p>
<p> The strand is polymerized from the 5' end to the 3' end. The nucleotide at the 5' end has a free phosphate and the nucleotide at the 3' end has a free 3'OH group on its sugar. </p>
<h3> <b> Nomenclature </b> </h3>
<p> S-P backbone is not written, just the bases and the polarity of the strand. <br /> <em><b> ALWAYS write 5’ and 3’ on each nucleic acid strand! </b></em>
<p> In other words, written nucleic acids take the following form: <br /> <h3>5'B<sub>1</sub>B<sub>2</sub>B<sub>3</sub>B<sub>4</sub>B<sub>5</sub>B<sub>6</sub>3'</h3> <br />
<p> For example: </p><h3>5'GAATCC3'</h3>
<br />
<h3> <b> Polarity of Synthesis </b> </h3>
<p> Nucleic acids are made from 5’P to 3’OH. This means that the –3’OH end is the growing end, i.e. receives the incoming nucleotide, as shown below. <br /><br /> <b> Base order = INFORMATION <br /> Polarity = 5’ and 3’ ends show first to last nucleotide added and confer a direction to read information</b></p>
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<h2 class="hd hd-2 unit-title">Exercise 1 (Labeling and Building Nucleic Acids)</h2>
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<p>Which of the following correctly demonstrates how to label the 5' end of the nucleic acid below?<br/>Select the best answer.</p>
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<b> TTCCGG3'</b>
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Question 3
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<p>Click on the image below to to place the circle at the part of the polymer where the incoming A nucleotide (deoxyadenosine triphosphate) will be added.</p>
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<h2 class="hd hd-2 unit-title">Exercise 2 (Interpreting Nucleic Acids)</h2>
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Question 1
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<p><b>A:</b> 5' AGCATG 3' </p>
<p><b>B:</b> 5' GTACGA 3'</p>
<p><b>C:</b> 3' GTACGA 5'</p>
<br/>
<p>Which of the following sets of DNA molecules (shown above) contain the same information? Select the best answer.</p>
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