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<h2 class="hd hd-2 unit-title">Reference Summary</h2>
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<h3><b>Transcription</b></h3>
<p> An RNA molecule is synthesized from one strand of a dsDNA; this strand used for synthesis is called the <strong>template strand</strong>. </p><p>Like DNA, RNA is synthesized 5' to 3'. </p><p>Once finished, the RNA transcript dissociates from the template strand, and the two DNA strands hybridize again to form dsDNA.</p>
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<img src='/assets/courseware/v1/70f34ce5ec809f12c0a99d03a0251460/asset-v1:OCW+Pre-7.01+1T2020+type@asset+block/n0_newBW.png' alt="This figure shows the process of transcription. The figure is divided by dashed lines into three sections. The first section is captioned: gene (d s D N A). Here, a double stranded D N A is shown as a pair of horizontal black lines. The top line is labelled as the non-template strand with a 5 prime label on its left end and a 3 prime label on its right end. The bottom line is labelled as the template strand with a 3 prime label on its left end and a 5 prime label on its right end. There is a thick arrow spanning a dashed line to mark the transition to the second section. The two DNA strands are repeated in the second section, although now they are separated by a larger distance. There is a gray arrow that points from a 5 prime label on its left end to a 3 prime label on its right end. This gray arrow is aligned on just above the bottom D N A strand, which is the template strand and runs from 3 prime on the left to 5 prime on the right. Below the bottom D N A strand is a bracket that spans the length and location of the gray arrow. The bracket is labelled as the transcribed region. Furthermore, the left side of the bracket is labelled as the start site and the right side of the bracket is labelled as the stop site. This second section has the following caption: The template is copied from 3 prime to 5 prime, and the RNA is synthesized 5 prime to 3 prime. There is another thick arrow spanning a dashed line to mark the transition to the third and final section. This section is captioned: R N A (transcript). The gray arrow from before is copied in this third section as a gray line and is labelled 5 prime on its left end and 3 prime on its right end.">
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<h2 class="hd hd-2 unit-title">Exercise 1 (Transcribing DNA 1)</h2>
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<p>The following RNA strand was produced by transcription of the gene shown in Question 1.</p>
<p><center><font size="+4"><strong> 5' GUGUCGGUUAGC 3' </strong></font></center></p>
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<p>Select the DNA strand that was used as the template strand to produce the RNA above. <em> (RNA is complementary to the template strand.)</em></p>
<p><img src="/assets/courseware/v1/e4adae8cfccf0eb7a0a67d0adf285e61/asset-v1:OCW+Pre-7.01+1T2020+type@asset+block/n2.jpg" width="600" style="float: center;" alt="Double stranded D N A. The top strand reads 5 prime G T G T C G G T T A G C 3 prime. The bottom strand reads 3 prime C A C A G C C A A T C G 5 prime."/></p>
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<p>Which direction was the template strand <b>transcribed</b> to create the RNA? <em> (Nucleic acids are synthesized 5&#8217; &#8594; 3&#8217;.) </em> </p>
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<h2 class="hd hd-2 unit-title">Exercise 2 (Transcribing DNA 2)</h2>
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<p>The following RNA strand was produced by transcription of the gene shown in Question 1.</p>
<p><center><font size="+4"><strong> 3' CCUUAUCG 5' </strong></font></center></p>
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Question 1
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<p>Select the DNA strand that was used as the template strand to produce the RNA above. <em> (RNA is complementary to the template strand.)</em></p>
<p><img src="/assets/courseware/v1/5cb4d85d699742381f226b3346bc1761/asset-v1:OCW+Pre-7.01+1T2020+type@asset+block/n4.png" width="600" style="float: center;" alt="Double stranded D N A. The top strand reads 5 prime G G A A T A G C 3 prime. The bottom strand reads 3 prime C C T T A T C G 5 prime."/></p>
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<p>Which direction was the template strand <b>transcribed</b> to create the RNA? <em> (Nucleic acids are synthesized 5&#8217; &#8594; 3&#8217;.) </em> </p>
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