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<h2 class="hd hd-2 unit-title">Lecture 3.2</h2>
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<h2 class="hd hd-2 unit-title">Reference Summary</h2>
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<h3><strong> The steps of DNA Replication </strong></h3>
<p>Step 1. The two strands of the original DNA separate. </p>
<img src='/assets/courseware/v1/4bc57f274abc7a794250a1bc9a9bf222/asset-v1:OCW+Pre-7.01+1T2020+type@asset+block/m0_1.png' alt="This image depicts double stranded D N A being separated into two pieces of single stranded D N A. The top strand represented as a black arrow that starts at the 5 prime end and ends at the 3 prime end. A sequence is given in the middle of the arrow. The sequence is G A T T A C A. The bottom strand is complementary and antiparallel. The bottom strand also represented as a black arrow that goes from the 5 prime end to the 3 prime end; the 3 prime end is on the left and the 5 prime end is on the right. The sequence of this strand is C T A A T G T. This double stranded D N A has two thick arrows that show it being separated into two single stranded molecules. The top strand is shown on the left and the bottom strand is shown on the right. ">
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<p>Step 2. RNA primers attach to the separated DNA strands. </p>
<img src='/assets/courseware/v1/319a9ebe0a0925672cf0ac2ff2a5438f/asset-v1:OCW+Pre-7.01+1T2020+type@asset+block/m0_2.png' alt="This image depicts R N A primers attaching to the single stranded D N A strands. At the left, the D N A sequence is represented as a black arrow that starts at the 5 prime end and ends at the 3 prime end. This strand has the following sequence: G A T T A C A. The D N A strand is labelled as a template. There is a short gray arrow that is aligned at the bottom right of the D N A template. This gray arrow is labelled as an R N A primer. The gray arrow goes from the 5 prime end to the 3 prime end but the 3 prime end is on the left and the 5 prime end is on the right. In this way, the 5 prime end of the R N A primer is aligned with and attached to the 3 prime end of the D N A template. At the right, the other D N A sequence is represented as a black arrow that goes from the 5 prime end to the 3 prime end; the 3 prime end is on the left and the 5 prime end is on the right. This strand has the following sequence: C T A A T G T. The D N A strand is labelled as a template. Just as before, there is a short gray arrow that is aligned at the top left of the D N A template. This gray arrow is also labelled as an R N A primer. The gray arrow goes from the 5 prime end at the left to the 3 prime end at the right. In this way, the 5 prime end of the R N A primer is aligned with and attached to the 3 prime end of the D N A template.">
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<p>Step 3. The DNA is replicated off of the 3’ end of the RNA primers.</p>
<img src='/assets/courseware/v1/02dbcc2f4ff227938abdf49e01f463bd/asset-v1:OCW+Pre-7.01+1T2020+type@asset+block/m0_3.png' alt="This image depicts the synthesis of new D N A via extention of the R N A primers that are attached to the single stranded D N A strands. The diagram starts with a replication of the figure from step 2. The description of this first part of the diagram is repeated here. At the left, the D N A sequence is represented as a black arrow that starts at the 5 prime end and ends at the 3 prime end. This strand has the following sequence: G A T T A C A. The D N A strand is labelled as a template. There is a short gray arrow that is aligned at the bottom right of the D N A template. This gray arrow is labelled as an R N A primer. The gray arrow goes from the 5 prime end to the 3 prime end but the 3 prime end is on the left and the 5 prime end is on the right. In this way, the 5 prime end of the R N A primer is aligned with and attached to the 3 prime end of the D N A template. At the right, the other D N A sequence is represented as a black arrow that goes from the 5 prime end to the 3 prime end; the 3 prime end is on the left and the 5 prime end is on the right. This strand has the following sequence: C T A A T G T. The D N A strand is labelled as a template. Just as before, there is a short gray arrow that is aligned at the top left of the D N A template. This gray arrow is also labelled as an R N A primer. The gray arrow goes from the 5 prime end at the left to the 3 prime end at the right. In this way, the 5 prime end of the R N A primer is aligned with and attached to the 3 prime end of the D N A template. Now, there are two thick arrows that show each of these D N A and R N A pairs being converted to new double stranded D N A molecules. The product from the left template is a double stranded D N A where the top strand is black and bears the sequence 5 prime G A T T A C A 3 prime. This strand was from the original double stranded D N A. The bottom strand is gray and bears the sequence 3 prime C T A A T G T 5 prime. This strand is newly synthesized. The product from the right template is a double stranded D N A where the top strand is gray and bears the sequence 5 prime G A T T A C A 3 prime. This strand is newly synthesized. The bottom strand is black and bears the sequence 3 prime C T A A T G T 5 prime. This strand was from the original double stranded D N A.">
<p>Thus, the result of DNA replication is two semiconservative, identical DNA molecules. </p>
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<h2 class="hd hd-2 unit-title">Exercise 1 (DNA Synthesis)</h2>
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<p>The following 2 questions center around the double-stranded DNA shown below. </p>
<img src='/assets/courseware/v1/611ebb8fafe0a85d243d2d8018f57308/asset-v1:OCW+Pre-7.01+1T2020+type@asset+block/m1.png' alt="This image shows the sequences of two complementary strands of D N A. The top strand is written as 5 prime C A T 3 prime. The bottom strand is written as 3 prime G T A 5 prime.">
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Question 2
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<div class="wrapper-problem-response" tabindex="-1" aria-label="Question 1" role="group"><p>Select the products of replicating the DNA molecule shown above.</p>
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<input type="radio" name="input_3daf01dfaebb43bc8cc95f35a594131e_2_1" id="input_3daf01dfaebb43bc8cc95f35a594131e_2_1_choice_0" class="field-input input-radio" value="choice_0"/><label id="3daf01dfaebb43bc8cc95f35a594131e_2_1-choice_0-label" for="input_3daf01dfaebb43bc8cc95f35a594131e_2_1_choice_0" class="response-label field-label label-inline" aria-describedby="status_3daf01dfaebb43bc8cc95f35a594131e_2_1"> <img src="/assets/courseware/v1/59ba34281149fa27cff622c4935968ea/asset-v1:OCW+Pre-7.01+1T2020+type@asset+block/3.2_ex1_q2_a.png" width="200" style="float:center;" alt="One double stranded product of D N A replication. The top strand reads 5 prime C A T 3 prime. The bottom strand reads 3 prime G T A 5 prime."/>
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<input type="radio" name="input_3daf01dfaebb43bc8cc95f35a594131e_2_1" id="input_3daf01dfaebb43bc8cc95f35a594131e_2_1_choice_1" class="field-input input-radio" value="choice_1"/><label id="3daf01dfaebb43bc8cc95f35a594131e_2_1-choice_1-label" for="input_3daf01dfaebb43bc8cc95f35a594131e_2_1_choice_1" class="response-label field-label label-inline" aria-describedby="status_3daf01dfaebb43bc8cc95f35a594131e_2_1"> <img src="/assets/courseware/v1/e7792c11aec1d66a3644a59dbdae1571/asset-v1:OCW+Pre-7.01+1T2020+type@asset+block/3.2_ex1_q2_b.png" width="200" style="float:center;" alt="One double stranded product of D N A replication. The top strand reads 5 prime C A T 3 prime. The bottom strand reads 3 prime C A T 5 prime."/>
</label>
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<input type="radio" name="input_3daf01dfaebb43bc8cc95f35a594131e_2_1" id="input_3daf01dfaebb43bc8cc95f35a594131e_2_1_choice_2" class="field-input input-radio" value="choice_2"/><label id="3daf01dfaebb43bc8cc95f35a594131e_2_1-choice_2-label" for="input_3daf01dfaebb43bc8cc95f35a594131e_2_1_choice_2" class="response-label field-label label-inline" aria-describedby="status_3daf01dfaebb43bc8cc95f35a594131e_2_1"> <img src="/assets/courseware/v1/79d5ae5e18858d5f9eeb65fd5ff2a508/asset-v1:OCW+Pre-7.01+1T2020+type@asset+block/3.2_ex1_q2_c.png" width="200" style="float:center;" alt="One double stranded product of D N A replication. The top strand reads 5 prime G T A 3 prime. The bottom strand reads 3 prime G T A 5 prime."/>
</label>
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<input type="radio" name="input_3daf01dfaebb43bc8cc95f35a594131e_2_1" id="input_3daf01dfaebb43bc8cc95f35a594131e_2_1_choice_3" class="field-input input-radio" value="choice_3"/><label id="3daf01dfaebb43bc8cc95f35a594131e_2_1-choice_3-label" for="input_3daf01dfaebb43bc8cc95f35a594131e_2_1_choice_3" class="response-label field-label label-inline" aria-describedby="status_3daf01dfaebb43bc8cc95f35a594131e_2_1"> <img src="/assets/courseware/v1/0ca8b2b37935aa173b40500f409dff00/asset-v1:OCW+Pre-7.01+1T2020+type@asset+block/3.2_ex1_q2_d.png" width="200" style="float:center;" alt="Two double stranded products of D N A replication. The top strand of the first pair reads 5 prime C A T 3 prime. The bottom strand of the first pair reads 3 prime G T A 5 prime. The top strand of the second pair reads 5 prime C A T 3 prime. The bottom strand of the second pair reads 3 prime G T A 5 prime."/>
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<input type="radio" name="input_3daf01dfaebb43bc8cc95f35a594131e_2_1" id="input_3daf01dfaebb43bc8cc95f35a594131e_2_1_choice_4" class="field-input input-radio" value="choice_4"/><label id="3daf01dfaebb43bc8cc95f35a594131e_2_1-choice_4-label" for="input_3daf01dfaebb43bc8cc95f35a594131e_2_1_choice_4" class="response-label field-label label-inline" aria-describedby="status_3daf01dfaebb43bc8cc95f35a594131e_2_1"> <img src="/assets/courseware/v1/97eb13acbd2519c8298fbcd90c427890/asset-v1:OCW+Pre-7.01+1T2020+type@asset+block/3.2_ex1_q2_e.png" width="200" style="float:center;" alt="Two double stranded products of D N A replication. The top strand of the first pair reads 5 prime C A T 3 prime. The bottom strand of the first pair reads 3 prime C A T 5 prime. The top strand of the second pair reads 5 prime G T A 3 prime. The bottom strand of the second pair reads 3 prime G T A 5 prime."/>
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Question 3
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<div class="wrapper-problem-response" tabindex="-1" aria-label="Question 1" role="group"><p>In what direction does DNA synthesis proceed for the RNA primer in the figure below? Select the best answer. </p>
<p><img src="/assets/courseware/v1/70d786a607dac26f9689454877e64f90/asset-v1:OCW+Pre-7.01+1T2020+type@asset+block/m3.png" width="600" style="float:center;" alt="Two nucleic acid strands. The top strand is the D N A template and reads 5 prime G C A T T T T A A C C G G A A 3 prime. The bottom strand is the R N A primer and reads A A U U G 5 prime."/></p>
<div class="choicegroup capa_inputtype" id="inputtype_bf2faa1f753d4c8f828db9e2a9b24176_2_1">
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<input type="radio" name="input_bf2faa1f753d4c8f828db9e2a9b24176_2_1" id="input_bf2faa1f753d4c8f828db9e2a9b24176_2_1_choice_0" class="field-input input-radio" value="choice_0"/><label id="bf2faa1f753d4c8f828db9e2a9b24176_2_1-choice_0-label" for="input_bf2faa1f753d4c8f828db9e2a9b24176_2_1_choice_0" class="response-label field-label label-inline" aria-describedby="status_bf2faa1f753d4c8f828db9e2a9b24176_2_1"> from left to the right (extending the 5' end of the primer)
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<input type="radio" name="input_bf2faa1f753d4c8f828db9e2a9b24176_2_1" id="input_bf2faa1f753d4c8f828db9e2a9b24176_2_1_choice_1" class="field-input input-radio" value="choice_1"/><label id="bf2faa1f753d4c8f828db9e2a9b24176_2_1-choice_1-label" for="input_bf2faa1f753d4c8f828db9e2a9b24176_2_1_choice_1" class="response-label field-label label-inline" aria-describedby="status_bf2faa1f753d4c8f828db9e2a9b24176_2_1"> from right to the left (extending the 3' end of the primer)
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Question 4
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<p> Write below the next 5 bases that will be added to the sequence from the figure in the question above. <br/> Be sure to indicate polarity! (<b>Ex:</b> 5'XXXX3' or 3'XXXX5')</p>
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<h2 class="hd hd-2 unit-title">Exercise 2 (RNA Primers)</h2>
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<p>The following questions center around the following DNA template sequence. </p>
<!--<img src='/assets/courseware/v1/e4ee9e22744d781fddd946f0acdde0d3/asset-v1:OCW+Pre-7.01+1T2020+type@asset+block/m4.png' alt="This image shows the sequence of a strand of D N A. The sequence is written as 5 prime G T G T C G G T T A C G 3 prime." >-->
<p><center><font size="+4"><strong> 5' GTGTCGGTTACG 3' </strong></font></center></p>
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Question 1
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<p> Which of the RNA primers below would allow replication of the template?
<br/>Check all that apply.</p>
<div class="wrapper-problem-response" tabindex="-1" aria-label="Question 1" role="group"><div class="choicegroup capa_inputtype" id="inputtype_b8be5bab44084087a881b7a63082026e_2_1">
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<input type="checkbox" name="input_b8be5bab44084087a881b7a63082026e_2_1[]" id="input_b8be5bab44084087a881b7a63082026e_2_1_choice_0" class="field-input input-checkbox" value="choice_0"/><label id="b8be5bab44084087a881b7a63082026e_2_1-choice_0-label" for="input_b8be5bab44084087a881b7a63082026e_2_1_choice_0" class="response-label field-label label-inline" aria-describedby="status_b8be5bab44084087a881b7a63082026e_2_1"> 5' CGGUU 3'
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<input type="checkbox" name="input_b8be5bab44084087a881b7a63082026e_2_1[]" id="input_b8be5bab44084087a881b7a63082026e_2_1_choice_1" class="field-input input-checkbox" value="choice_1"/><label id="b8be5bab44084087a881b7a63082026e_2_1-choice_1-label" for="input_b8be5bab44084087a881b7a63082026e_2_1_choice_1" class="response-label field-label label-inline" aria-describedby="status_b8be5bab44084087a881b7a63082026e_2_1"> 5' CGUAA 3'
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<input type="checkbox" name="input_b8be5bab44084087a881b7a63082026e_2_1[]" id="input_b8be5bab44084087a881b7a63082026e_2_1_choice_2" class="field-input input-checkbox" value="choice_2"/><label id="b8be5bab44084087a881b7a63082026e_2_1-choice_2-label" for="input_b8be5bab44084087a881b7a63082026e_2_1_choice_2" class="response-label field-label label-inline" aria-describedby="status_b8be5bab44084087a881b7a63082026e_2_1"> 5' GACAC 3'
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<p>Note: Make sure you select <i>all</i> correct options!</p>
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Question 2
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<p> Write below the bases of the <b>template DNA</b> that the primer will hybridize to. <br/> Be sure to indicate polarity! (<b>Ex:</b> 5'XXXX3' or 3'XXXX5')</p>
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